| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1001 | Holdemania filiformis | TGGAAGAACGGTTTGCGC | ATTCGTTGCCCTGCGGAT | 58.97 | 59.73 | 261 | 48 |
100.00%
|
50.00%
|
| Primer 1002 | Holdemania filiformis | ACTGCGCAACGGTCAGAT | TCGCTGTGATGTCGCCTT | 59.66 | 59.34 | 191 | 48 |
100.00%
|
50.00%
|
| Primer 1003 | Holdemania filiformis | AACTGCGCAACGGTCAGA | TTCGCTGTGATGTCGCCTT | 59.89 | 60.01 | 193 | 48 |
100.00%
|
50.00%
|
| Primer 1004 | Holdemania filiformis | ATGCCTGTTCGACGCCTT | GCAGCCGTCCAGAAAGGTA | 59.65 | 59.70 | 193 | 48 |
100.00%
|
50.00%
|
| Primer 1005 | Holdemania filiformis | ACGCTGCTCAACTGCATCT | AATTGTCAACGGCAGCGC | 60.00 | 59.74 | 195 | 48 |
100.00%
|
50.00%
|
| Primer 1006 | Holdemania filiformis | AGACGACGCTGCTCAACT | AATTGTCAACGGCAGCGC | 58.96 | 59.74 | 200 | 48 |
100.00%
|
50.00%
|
| Primer 1007 | Holdemania filiformis | AACGACGCTGCGGATCAA | CATAAATGCCGAGCACCGC | 60.05 | 60.01 | 194 | 48 |
100.00%
|
50.00%
|
| Primer 1008 | Holdemania filiformis | TGCAGGAAGTTCTGAGCCG | TCAGTTCGTCGTGCGCTT | 60.00 | 59.97 | 291 | 48 |
100.00%
|
50.00%
|
| Primer 1009 | Holdemania filiformis | AAGGCATGTTGGATGCGC | TCTGCACGTCGTCGCAAT | 59.11 | 60.05 | 261 | 48 |
100.00%
|
50.00%
|
| Primer 1010 | Holdemania filiformis | AAACGATTGTCGCTGCGC | CCGTTCATCCTGCAGCTGA | 59.83 | 60.08 | 158 | 48 |
100.00%
|
50.00%
|